Product Overview

The A1 vs A2 Milk PCR Kit is an advanced molecular diagnostic tool designed to identify and differentiate between A1 and A2 beta-casein genetic variants in bovine milk. This kit enables rapid classification of milk based on its genetic composition, supporting dairy producers, processors, and retailers in meeting consumer demands for A2 milk products and ensuring accurate labeling and traceability.

Key Features

• Precise Genetic Variant Identification – Targets specific SNP (Single Nucleotide Polymorphism) markers that distinguish A1 from A2 beta-casein alleles

• High Accuracy – >99% sensitivity and specificity for reliable variant detection

• Rapid Turnaround – Complete results within 2-3 hours from sample preparation

• Simple Workflow – Minimal sample preprocessing required; compatible with standard PCR equipment

• Cost-Effective Solution – Economical screening for large-scale dairy operations

• Scalable Testing – Suitable for individual cow testing or bulk milk screening

Applications

• Classification of dairy cattle herds for A2 milk production

• Quality assurance and product authentication in A2 milk supply chains

• Genetic screening for selective breeding programs

• Regulatory compliance and consumer transparency

• Verification of A2 milk claims on dairy products

• Research studies on milk protein variants and nutritional properties

Kit Contents

• PCR Master Mix with A1/A2-specific primers

• Positive Control DNA (A1 and A2 variants)

• Negative Control

• DNA extraction buffer and reagents

• Detailed protocol with interpretation guidelines

Specifications

• Sample Type: Milk (raw or pasteurized), or cattle blood/tissue

• Detection Sensitivity: Identifies both homozygous (A1A1, A2A2) and heterozygous (A1A2) genotypes

• Result Format: Distinct PCR band patterns for easy visual interpretation

• Shelf Life: 12 months at 2-8°C when stored as directed

• Kit Format: 10, 50 100 reactions per kit

Scientific Background

Beta-casein is a major milk protein with two primary genetic variants: A1 and A2. The A1 variant produces beta-casomorphin-7 (BCM-7) during digestion, while the A2 variant does not. Growing consumer interest in A2 milk—driven by perceived digestive and health benefits—has created demand for accurate genetic identification and market differentiation.

Why Choose Kiagene Biosciences?

Our A1 vs A2 Milk PCR Kit is developed using validated molecular protocols, optimized for Indian dairy breeds. Our technical team provides comprehensive support for implementation and result interpretation, ensuring seamless integration into your laboratory workflows.