Recombinant Protein A – High-Purity Immunoaffinity Ligand for Antibody Purification

Product Overview

Recombinant Protein A is a highly purified, engineered bacterial protein derived from Staphylococcus aureus, specifically designed for selective binding and purification of immunoglobulins (antibodies). This recombinant variant offers superior consistency, batch-to-batch reliability, and scalability compared to native Protein A, making it an essential reagent for antibody production, purification, and diagnostic applications across research, biotechnology, and pharmaceutical industries.

Key Features

• High Binding Affinity – Specifically binds the Fc region of IgG antibodies with exceptional selectivity

• Recombinant Expression – Produced in E. coli for consistent quality and reduced endotoxin levels

• Superior Purity – >95% purity, ensuring minimal non-specific interactions

• Batch Consistency – Standardized production protocols guarantee reproducible results across batches

• Stable & Long Shelf Life – Maintains activity for extended periods under proper storage conditions

• Versatile Applications – Compatible with multiple purification methodologies and scale-up processes

Applications

• Affinity chromatography for monoclonal and polyclonal antibody purification

• Antibody immobilization on solid supports (agarose, magnetic beads, biosensors)

• Diagnostic assay development (ELISA, lateral flow assays, immunosensors)

• Therapeutic antibody manufacturing and process development

• Immunoprecipitation and co-immunoprecipitation (Co-IP) experiments

• Antibody quantification and characterization studies

• Development of protein A columns and affinity matrices

Binding Characteristics

• Target: IgG antibodies (human, mouse, rabbit, goat, and other mammalian species)

• Binding Specificity: Fc region of immunoglobulins

• Binding Capacity: Typically 10-15 mg IgG per mL of Protein A immobilized on standard matrices

• pH Stability: Functional across pH 4.0-8.0 range

• Elution: Low pH buffers (pH 2.5-3.0) or high salt conditions

Product Specifications

• Source: Recombinantly expressed in E. coli

• Purity: ≥95% (as determined by SDS-PAGE)

• Endotoxin Level: <1 EU/mg (suitable for clinical and pharmaceutical use)

• Molecular Weight: ~56 kDa (native Protein A monomer)

• Form: Liquid solution or lyophilized powder (specify preference)

• Concentration: Customizable (typically supplied at 1-10 mg/mL)

• Shelf Life: 24 months at -20°C (liquid) or 36 months at 2-8°C (lyophilized)

• Storage Buffer: PBS or custom buffer options available

Available Formats

• Protein A Solution – Ready-to-use liquid format for immediate applications

• Protein A Lyophilized – Freeze-dried powder for long-term storage and custom reconstitution

• Bulk Supply – Large-scale quantities for manufacturing and process development

Quality Assurance

• SDS-PAGE analysis confirming purity and molecular weight

• Endotoxin testing (LAL assay) for pharmaceutical-grade applications

• Functional assay demonstrating IgG binding capacity

• Sterility testing (where applicable)

• Certificate of Analysis provided with each batch

Technical Support

Our team provides comprehensive technical guidance on:

• Protocol optimization for antibody purification workflows

• Selection of appropriate Protein A formats for your application

• Scale-up considerations for manufacturing processes

• Troubleshooting and performance optimization

Why Choose Kiagene Biosciences?

Kiagene Biosciences supplies pharmaceutical-grade Recombinant Protein A manufactured under strict quality control standards. Our recombinant production methodology ensures superior batch consistency, reduced contamination risks, and reliable performance across research, diagnostic, and therapeutic applications. We are committed to supporting your antibody purification and immunodiagnostic needs with high-quality, cost-effective solutions.

Custom Options Available

We offer customization including alternative buffer systems, conjugation to specific matrices, bulk quantities, and format modifications to meet your specific research or manufacturing requirements. Contact our technical team for tailored solutions.