Recombinant Protein G – Broad-Spectrum Immunoglobulin Binding Ligand for Antibody Purification

Product Overview

Recombinant Protein G is a highly purified, engineered bacterial protein derived from Streptococcus species, specifically designed for selective binding and purification of a wider range of immunoglobulins across diverse species. This recombinant variant provides superior versatility compared to native Protein G, enabling purification of antibodies that may have limited binding to Protein A, while maintaining exceptional consistency, batch-to-batch reliability, and scalability for research, diagnostic, and pharmaceutical applications.

Key Features

• Broad Antibody Specificity – Binds IgG and other immunoglobulin classes across multiple species with high affinity

• Enhanced Species Coverage – Particularly effective for antibodies with suboptimal Protein A binding (e.g., certain mouse IgG subclasses, human IgG3)

• Recombinant Expression – Produced in E. coli for consistent quality, reduced endotoxin levels, and improved purity

• Superior Consistency – Standardized production protocols guarantee reproducible results across all batches

• High Binding Capacity – Excellent immobilization efficiency on chromatography matrices and solid supports

• Extended Shelf Life – Maintains full activity for extended periods under appropriate storage conditions

• Complementary to Protein A – Ideal for dual-purification strategies or antibodies with mixed-species origins

Applications

• Affinity chromatography for monoclonal and polyclonal antibody purification

• Purification of antibodies with limited Protein A binding

• Antibody immobilization on agarose, magnetic beads, and biosensor surfaces

• Diagnostic assay development (ELISA, lateral flow assays, immunosensors)

• Therapeutic antibody manufacturing and process optimization

• Immunoprecipitation and co-immunoprecipitation (Co-IP) experiments

• Antibody quantification, characterization, and stability studies

• Development of Protein G columns and affinity matrices

• Research applications requiring broad immunoglobulin specificity

Binding Characteristics

• Primary Targets: IgG, IgA, IgM, and IgE immunoglobulins

• Species Coverage: Human, mouse, rabbit, goat, sheep, guinea pig, and other mammalian species

• Binding Region: Fc and Fab regions of immunoglobulins (broader recognition than Protein A)

• Binding Capacity: Typically 15-20 mg IgG per mL of Protein G immobilized on standard matrices

• pH Stability: Functional across pH 4.0-8.5 range

• Elution: Low pH buffers (pH 2.5-3.0), high salt conditions, or mild organic solvents

Product Specifications

• Source: Recombinantly expressed in E. coli

• Purity: ≥95% (as determined by SDS-PAGE)

• Endotoxin Level: <1 EU/mg (suitable for clinical and pharmaceutical applications)

• Molecular Weight: ~65 kDa (native Protein G monomer)

• Form: Liquid solution or lyophilized powder (specify preference)

• Concentration: Customizable (typically supplied at 1-10 mg/mL)

• Shelf Life: 24 months at -20°C (liquid) or 36 months at 2-8°C (lyophilized)

• Storage Buffer: PBS or custom buffer options available

Available Formats

• Protein G Solution – Ready-to-use liquid format for immediate applications

• Protein G Lyophilized – Freeze-dried powder for long-term storage and custom reconstitution

• Protein G Conjugates – Pre-conjugated with agarose, magnetic beads, or other solid supports (custom options available)

• Bulk Supply – Large-scale quantities for manufacturing and process development

Quality Assurance

• SDS-PAGE analysis confirming purity and molecular weight

• Endotoxin testing (LAL assay) for pharmaceutical-grade applications

• Functional assay demonstrating IgG binding capacity across species

• Antibody cross-reactivity panel testing

• Certificate of Analysis provided with each batch

Protein A vs Protein G Comparison

Technical Support

Our team provides comprehensive guidance on:

• Protocol optimization for diverse antibody types and species

• Selection of appropriate Protein G formats for your application

• Dual purification strategies combining Protein A and Protein G

• Scale-up considerations for manufacturing processes

• Troubleshooting and performance optimization

Why Choose Kiagene Biosciences?

Kiagene Biosciences supplies pharmaceutical-grade Recombinant Protein G manufactured under rigorous quality control standards. Our recombinant production methodology ensures superior batch consistency, minimal batch-to-batch variation, and reliable performance across research, diagnostic, and therapeutic applications. Whether you're working with challenging antibody species or require complementary purification capabilities, our Protein G delivers consistent, high-quality results.

Custom Options Available

We offer customization including alternative buffer systems, conjugation to specific matrices, bulk quantities, species-specific formulations, and format modifications tailored to your unique research or manufacturing requirements. Contact our technical team for customized solutions.